site-directed mutagenesis
genetics
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work of Smith
- In Michael Smith
…by a technique known as site-directed mutagenesis. The approach was revolutionary because it enabled researchers to introduce specific mutations into genes by simply synthesizing oligonucleotides (short chains of nucleotides, the building blocks of DNA) that are complementary to the target DNA sequence and that include the desired mutation. For his
Read More - In genetics: Recombinant DNA technology and the polymerase chain reaction
…creating a technique known as site-directed mutagenesis. In 1983 American biochemist Kary B. Mullis invented the polymerase chain reaction, a method for rapidly detecting and amplifying a specific DNA sequence without cloning it. In the last decade of the 20th century, progress in recombinant DNA technology and in the development…
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